Sample Preparation and Volumes Required for Lipid Assays
Serum or EDTA plasma should be removed from the red cells as soon as possible after collection and then frozen.
Tissue samples should be frozen in liquid nitrogen immediately after collection.
Complete the order submission form on line, make a copy, and place it in the container with your samples enclosed in a plastic bag if shipping.
Please label all tubes clearly and legibly.
Samples should be stored at -70°C. Heparin is not acceptable as an anticoagulant for lipid profiles. Samples with gross hemolysis or lipemia may yield false results.
| Assay | Requirements for Human/Canine | Requirements for Mouse/Rat |
|---|---|---|
| FPLC Lipoprotein Separation | 120 µl | 120 µl |
| Modification of LDL | Inquire | Inquire |
| Plasma Cholesterol | 15 µl serum or EDTA plasma | 15 µl serum or EDTA plasma |
| Plasma enzymatic FFA | 30 µl serum or EDTA plasma | 30 µl serum or EDTA plasma |
| Plasma HDL Cholesterol | 30 µl serum or EDTA plasma | 30 µl serum or EDTA plasma |
| Plasma Triglycerides | 15 µl serum or EDTA plasma | 15 µl serum or EDTA plasma |
| Tissue/Cell Cholesterol | ~100 mg tissue | ~100 mg tissue |
| Tissue/Cell Diglyceride | ~100 mg tissue | ~100 mg tissue |
| Tissue/Cell Phospholipid | ~100 mg tissue | ~100 mg tissue |
| Tissue/Cell Triglycerides | ~100 mg tissue | ~100 mg tissue |
| HDL Separation | Inquire | Inquire |
| LDL Separation | Inquire | Inquire |
Outside investigators should ship samples overnight on dry ice to:
1161 21st Avenue South
Room CC-3327 MCN
Nashville, TN 37232-2561
Please ship samples Monday through Thursday so that the core receives them no later than Friday of any given week. For additional information concerning Lipid Profiles, please contact Caroline Wiser at 615-343-2645.
